Phenotypic characterization of auxotrophic mutant of nontyphoidal Salmonella and determination of its cytotoxicity, tumor inhibiting cytokine gene expression in cell line models

An auxotrophic mutant of nontyphoidal Salmonella (NTS) strain (Salmonella Oslo) was phenotypically characterized in this study. The characterization based on phenotype, morphology, motility, biofilm forming ability, growth kinetics, etc. phenotypic results from the above experiments determined that showed variation characters wild-type strain. Subsequently, and NTS were subjected to epithelial cell invasion intracellular replication assays. real-time PCR analysis also performed analyse expression tumor inhibiting cytokine genes virulence post-bacterial infection lines. highest potential than whereas slower both Similar strain, retained cytotoxic when analysed vitro. Furthermore, proinflammatory such as TNF-? IL-1? upsurged with downregulation anti-inflammatory like TGF-?, IL-6 IL-10 post-infection In addition, pathogenicity island one two downregulated vitro except invA HeLa line. Therefore, positive attributes a antitumor agent terms expressing assessed Though study did not check inhibitory effect directly, findings emphasizes development novel less more immunogenic traits inhibit cells.